Urine Amino Acid Case Study - Eileen Durfee
Urine Amino Acid Case Study — Genetics + HTMA + Custom Transdermal Crèmes
How pairing DNA insights with urine amino acid testing guided two made-to-order crèmes—and moved Eileen from ~50% abnormal markers to a largely normalized profile.
- Serotonin:Dopamine ratio normalized
- NE:Epi ratio strengthened
- L-DOPA capacity restored
- Glycine & Beta-alanine calmed
- Sleep & stress response improved
Eileen’s Transformation
From chronic pain to wellness innovator — Eileen turned years of health challenges into practical solutions that help people restore energy, mobility, and mineral balance. The lab reports on this page are one chapter of that story; here is the bigger picture, in her own numbers.
Photos: ages 55 → 63 (2025) Body change: 183 lbs, size 14 → 150 lbs, size 6 Function Health biological age ~49.4 — about 13 years younger than her calendar age
Who is Eileen?
Eileen is a former nuclear power plant quality engineer who became ill from chemical exposure and spent years searching for answers. That journey led her to testing-based methods like Hair Tissue Mineral Analysis (HTMA), targeted mineral support, near-infrared sauna and detox practices — and ultimately to invent solutions that help others reclaim energy, mobility, and health. She practices what she teaches: the baseline and follow-up reports below are her own. And the story starts even earlier — her baby hair analysis, tested from samples saved in infancy, shows the toxic load she carried from the very beginning.
Why this test mattered
The Metabolic Testing (Urine) reads real-time chemistry—neurotransmitters, amino acids, and stress-hormone metabolites—through an HPA (stress) lens. With Eileen’s dense methylation/detox SNPs, this profile identified the exact co-factors and amino acids to deliver transdermally in day/night crèmes.
HPA summary
- Balanced HPA: decreases pro-inflammatory cytokines.
- Imbalanced HPA: drives inflammation; feedback loops drift.
Custom Transdermal Support AM + PM
AM crème (2×/day, ~30 days): catecholamine precursors/co-factors, active B-vitamins compatible with MTHFR/MTR/MTRR, gentle methyl donors.
PM crème (1×/night, ~55 days): inhibitory tone (GABA focus), histamine balance, sleep repair. Using 2×/night ≈ 30 days for stubborn insomnia.
Before (2022): ~50% abnormal markers
- 5-HT:DA ratio 0.36 (ref 0.49–1.67) — serotonergic tone low vs dopamine.
- NE:Epi ratio 3.30 — fragile sympathetic response.
- L-DOPA 3.90 (16.40–62.30) — limited dopamine synthesis capacity.
- Glycine 3433 (99.5–647.5) & Beta-alanine 121.5 (0.50–38.20) — high one-carbon/pyrimidine turnover demands.
After (2025): ratios normalized, hot spots cooled
- 5-HT:DA ratio 0.68 — in range; mood regulation steadier.
- NE:Epi ratio 6.19 — more resilient stress handling.
- L-DOPA 25.00 — dopamine synthesis capacity restored.
- Glycine 542 (now in range) & Beta-alanine 30.6 — detox “drag” markedly reduced.
- Asn/Ala/Orn/Histamine moved into range; Cystine remains lowish (12.9) → keep sulfur/antioxidant support.
Pathways at a glance
Genetics set the tendencies (COMT/MAO, MTHFR/MTR/MTRR, VDR, GST/NAT). The crèmes were tuned to the urine chemistry so the catecholaminergic/serotonergic, glutamatergic, and trans-methylation/trans-sulfuration pathways could flow instead of bottleneck.
What the profile analyzes
Creatinine; 5-HTP; Serotonin; Dopamine; Serotonin:Dopamine; Epinephrine; Norepinephrine; NE:Epi; 3-Methoxytyramine; L-DOPA; Normetanephrine; Metanephrine; GABA; Glutamic Acid; Histamine; aromatic/branched/essential amino acids (TRP, PHE, TYR, HIS, VAL, LEU, ILE, MET, THR, LYS, Hydroxylysine, PEA); non-essential amino acids (GLN, ASN, ASP, ALA, PRO, ARG, GLY, CYS, SER, Homocysteine, Cystathionine, Hydroxyproline, Homoserine, Homocystine, Cystine); non-proteinogenic (GABA, Beta-alanine, Histamine, Carnosine, Ornithine, Citrulline, Glutathione, Sarcosine, 2-Aminoadipic, Alpha-/Beta-aminobutyric acids).
Collection Essentials
10 AM mid-stream urine; limit protein the night before; avoid nuts, cheese, alcohol; no caffeine morning of.
AM Crème
2×/day (~30 days). Precursors + co-factors aligned to SNPs; gentle methyl donors.
PM Crème
1×/night (~55 days). Inhibitory tone, histamine/sleep support. 2×/night ≈ 30 days if needed.
Policy
Crèmes are made-to-order and non-returnable; no cancellations once compounded.
FAQs
What is Metabolic Testing (Urine)?
A comprehensive urine profile of amino acids, neurotransmitters, and stress metabolites with an HPA focus—used to tailor day/night transdermal crèmes.
How do the crèmes help?
They deliver targeted nutrients through the skin, smoothing kinetics for those with COMT/MAO/GST variants who may react to oral doses.
Who benefits?
Anyone optimizing stress response, inflammation, mood/sleep, detox comfort, or who wants chemistry that matches their genetics/HTMA.
Educational content only; not medical advice. Work with your practitioner for personalized guidance.
Next steps
Eileen’s results are her own experience; individual results vary. These statements have not been evaluated by the Food and Drug Administration. Our products are not intended to diagnose, treat, cure, or prevent any disease, and nothing on this page is a substitute for care from a qualified healthcare professional.
