Genetic Lab Testing with Consultation

3X4 Genetics Blueprint + Eileen Durfee Consultation

If it impacts your health, it belongs in your plan. The 3X4 Genetics Blueprint translates key genetic variants into clear, pathway-based health insights—covering 36 metabolic pathways across 6 health categories—so you can focus on the areas that matter most first.

What you receive

• Your pathway impact dashboard (from highest to lowest impact using easy color-coding) so priorities are obvious.
• Gene results organized by pathway, not dumped as raw data—so patterns appear (inflammation + detox + glucose control, etc.).
• A framework that connects your results to practical levers: nutrition, lifestyle, training, and recovery.

Why Eileen Durfee’s consultation makes it better

Many genetic reports are informative—but implementation is where people stall. The Blueprint itself emphasizes that the most useful insights emerge when genetic findings are combined with your history, diet, lifestyle, and goals in the hands of an expert practitioner.

With Eileen, you’re not just “reviewing genes.” You’re turning them into a personalized action plan, including:

• What to address first (highest-impact pathways)
• What’s likely driving your symptoms or plateaus (cross-pathway patterns)
• How to tailor food, supplements, stress, sleep, and training to your genetics
• Clear next steps you can actually follow

Why this approach is superior to typical testing

• Pathway-level interpretation (systems thinking): you see how genes interact inside real biology, not isolated SNP trivia.
• Prioritization built in: impact scoring + color-coding prevents overwhelm and guesswork.
• Practitioner-guided personalization: your results become a strategy, not a PDF that sits in a folder.

How many genes are tested?

137 genes/markers are included.

How these genes connect to pathways in 3X4 Genetics

In the 3X4 Genetics Blueprint, gene variants are organized into pathway-based results (so you’re not left with a disconnected SNP list). The report covers 36 metabolic pathways across 6 health categories, and it calculates an impact level for each pathway using color-coding to show where to focus first.

Below is a practical “gene → pathway” map (the same gene can influence more than one pathway).

Gene explanations by pathway

1) Cellular pathways

A) Methylation (methyl donors, one-carbon cycle, neurotransmitter balance)

Gene(s)	Explanation
MTHFR	folate activation (methyl cycle throughput)
MTHFD1	folate cycle support (one-carbon metabolism)
MTR	remethylates homocysteine to methionine
MTRR	regenerates active B12 for MTR
BHMT	alternate homocysteine “shortcut” using betaine/choline
CBS	diverts homocysteine toward transsulfuration (sulfur pathway)
TCN2	vitamin B12 transport into cells
PEMT	endogenous choline/phosphatidylcholine production
CHDH	choline → betaine (feeds BHMT methylation)

B) Inflammation (cytokines, acute phase response, immune signaling)

Gene(s)	Explanation
TNFA	inflammatory signaling intensity
IL-1	inflammatory activation signaling (marker)
IL-6 / IL-6R	cytokine signaling and receptor sensitivity
CRP	downstream inflammation/acute phase marker
HLA	immune recognition; links to gluten/autoimmune susceptibility
PPARA / PPARG / PPARD	metabolic inflammation switches (fat/glucose crosstalk)
SIRT1	inflammation control via energy-sensing pathways
FOXO1 / FOXO3	stress-response transcription factors

C) Oxidative stress (antioxidant enzymes, mitochondrial protection)

Gene(s)	Explanation
CAT	breaks down hydrogen peroxide
MNSOD (SOD2)	mitochondrial superoxide defense
GPX1	peroxide neutralization (glutathione system)
NQO1	quinone detox / antioxidant defense
NRF2	master “turn on antioxidant genes” regulator
OGG1	oxidative DNA repair
UCP1 / UCP2 / UCP3	mitochondrial uncoupling; ROS/energy balance

D) Detoxification (Phase I/II detox, clearance, xenobiotics)

Gene(s)	Explanation
Phase I (activation/processing)
CYP1A1 / CYP1A2 / CYP1B1 / CYP2C19 / CYP2C9 / CYP2D6 / CYP3A4	drug/hormone/toxin metabolism
EPHX1	epoxide detox (often paired with CYP activity)
ALDH2	aldehyde clearance (alcohol and oxidative byproducts)
Phase II (conjugation/clearance)
GSTM1 / GSTT1 / GSTP1 / GSTO2	glutathione conjugation capacity
NAT1 / NAT2	acetylation (amine/chemical handling)
SULT1A1	sulfation (hormones/phenols)
UGT2B15 / UGT2B17	glucuronidation (hormones/xenobiotics)
PON1	antioxidant/organophosphate handling (cardio + detox overlap)

2) Systems pathways

A) Collagen & joints (connective tissue structure + breakdown/repair)

Gene(s)	Explanation
COL1A1 / COL3A1 / COL12A1	collagen structural proteins
GDF5	joint/cartilage development and resilience
MMP1 / MMP2 / MMP3	collagen breakdown/remodeling enzymes
TIMP4	MMP inhibition (controls breakdown)
VEGFA	tissue perfusion/healing support (also vascular)

B) Glucose & insulin (glycemic regulation, insulin signaling, storage)

Gene(s)	Explanation
TCF7L2	insulin/glucose regulation risk signaling
IRS1	insulin receptor signaling efficiency
SLC2A2	glucose transport (GLUT2)
PPARG / PPARGC1A / PPARA / PPARD	insulin sensitivity + fuel switching
FTO	appetite/energy balance associations
MTNR1B	melatonin receptor; glucose timing/fasting glucose associations
ADIPOQ	adiponectin signaling; insulin sensitivity support
LEPR	leptin signaling; appetite + metabolic control

C) Memory & brain health (neuroplasticity, vascular brain support, aging risk)

Gene(s)	Explanation
APOE	lipid transport in brain; cognitive aging associations
TOMM40	mitochondrial import; cognition/aging associations
BDNF	neuroplasticity/learning; stress response
COMT	dopamine breakdown; cognition/stress tolerance overlap
CACNA1C	neuronal calcium signaling
AKT1	neuronal survival/signaling pathways
NOS3/ENOS	blood flow support (brain perfusion overlap)

D) Bone health (vitamin D axis + collagen structure)

Gene(s)	Explanation
VDR	vitamin D receptor function
GC	vitamin D binding/transport
CYP2R1	vitamin D activation step
COL1A1	bone matrix collagen backbone

E) Mood & behaviour (serotonin, dopamine, GABA, reward, bonding)

Gene(s)	Explanation
Serotonin signaling
5HT2A	serotonin receptor signaling (mood, sleep, perception)
HTR1A	serotonin receptor regulation (stress/mood)
Dopamine/reward
DRD1 / DRD3 / DRD4	dopamine receptors (motivation/novelty/reward)
ANKK1/DRD2	dopamine D2 pathway marker (reward/addiction tendency)
Breakdown enzymes / neurotransmitter balance
MAOA	monoamine breakdown (serotonin/dopamine/norepinephrine)
COMT	dopamine/norepinephrine breakdown (stress/cognition)
GAD1	makes GABA (calming neurotransmitter)
GABRA2	GABA receptor signaling (calm/anxiety traits)
Reward, stress buffering, connection
OPRM1	opioid receptor (reward/pain response)
FAAH	endocannabinoid breakdown (stress, reward, inflammation overlap)
OXTR	oxytocin receptor (bonding/social stress resilience)
ANK3 / CACNA1C	neuronal excitability/mood stability markers
CHRNA5	nicotinic receptor (stimulation/addiction tendency)

F) Hormone balance (estrogen/androgen metabolism + binding/clearance)

Gene(s)	Explanation
CYP17A1	sex steroid synthesis step
CYP19A1	aromatase (androgen → estrogen)
ESR2	estrogen receptor beta signaling
SHBG	hormone binding/availability
SRD5A1	androgen conversion (DHT pathway)
UGT2B15 / UGT2B17 / SULT1A1	hormone clearance (phase II)

G) Histamine overload (breakdown capacity)

Gene(s)	Explanation
DAO	histamine breakdown in gut/periphery
HNMT	histamine breakdown inside cells
(Also influenced by inflammation genes like IL-6/TNFA)

3) Cardiovascular health pathways

A) Vascular health (endothelial function, nitric oxide, repair)

Gene(s)	Explanation
NOS3 / ENOS	nitric oxide production; vessel flexibility
VEGF / VEGFA / VEGFR2	vascular growth/repair signaling
HO-1 (HMOX1)	oxidative/inflammatory vessel protection
CRP / OGG1 / NRF2	inflammation/oxidative stress impacts vessels

B) Blood pressure (RAAS signaling + adrenergic tone)

Gene(s)	Explanation
ACE / ACE2	angiotensin processing balance
AGT	angiotensinogen supply
AGTR1 / AGTR2	angiotensin receptor signaling
REN	renin initiation of RAAS cascade
ADRB2	adrenergic tone (stress response, vascular constriction/dilation)

C) Blood clotting (coagulation risk tendency)

Gene(s)	Explanation
F2	prothrombin pathway
F5	Factor V clotting regulation
HPA-1	platelet adhesion marker

D) Cholesterol & lipids (transport, triglycerides, HDL dynamics)

Gene(s)	Explanation
APOE	lipoprotein transport/clearance
APOA5 / APOC3	triglyceride handling
APOA2	fat response signaling (diet-fat sensitivity traits)
CETP	HDL/LDL particle exchange
LPL	triglyceride breakdown from particles
FABP2	intestinal fat absorption/handling
PON1	HDL antioxidant function

4) Energy pathways (fat storage, release, appetite, metabolism)

A) Pro-inflammatory fat

Gene(s)	Explanation
TNFA / IL-1 / IL-6 / IL-6R / CRP	adipose inflammation signaling
ADIPOQ	anti-inflammatory adipokine support
PPARG / PPARA / SIRT1	inflammation ↔ metabolism switches

B) Adipogenesis (fat-cell creation/storage propensity)

Gene(s)	Explanation
PPARG	adipocyte differentiation “master switch”
PLIN	fat droplet storage dynamics
ADRB3 / ADRB2	fat mobilization signaling
LEPR	leptin feedback (storage vs burn)
MMP2	tissue remodeling that can affect adipose expansion

C) Weight gain & weight loss resistance

Gene(s)	Explanation
FTO	weight regulation associations
MC4R	appetite/satiety control center signaling
CLOCK	circadian rhythm ↔ metabolism coupling
UCP1/2/3	energy expenditure/thermogenesis tendency
TCF7L2 / IRS1	glucose signaling that affects storage patterns

D) Exercise response & energy expenditure

Gene(s)	Explanation
PPARGC1A / PPARD / PPARA	mitochondrial biogenesis & fuel switching
ADRB2 / ADRB3	fat mobilization during activity
UCP genes	resting burn/thermogenesis traits

E) Appetite / satiety / intake

Gene(s)	Explanation
LEPR / MC4R / FTO	hunger/fullness signaling balance
TAS2R38	bitter taste sensitivity (food preference effects)

5) Activity pathways (training, performance, injury risk)

A) Injury / connective tissue recovery

Gene(s)	Explanation
COL1A1 / COL3A1 / COL12A1 / GDF5	tissue strength
MMP3 / TIMP4	remodeling balance
VEGFA	healing blood supply support
TNFA / CRP	inflammation that can impair recovery

B) Endurance / oxygen utilization

Gene(s)	Explanation
ACE	endurance vs power tendency marker
HIF1A	oxygen response signaling
NOS3	blood flow support
VEGF	capillary growth signaling
PPARGC1A / PPARA	aerobic capacity support pathways

C) Power / sprint traits

Gene(s)	Explanation
ACTN3	fast-twitch muscle performance marker
CKM	muscle energy buffering
AMPD1	ATP recycling during intense bursts

D) Training response (adaptation rate)

Gene(s)	Explanation
ACE / ACTN3 / PPARGC1A / NRF2	adaptation, recovery, oxidative load handling
ACSL1	fatty acid activation for energy use in muscle

6) Nutrients pathways (absorption, activation, utilization)

A) Vitamin B12

Gene(s)	Explanation
TCN2	transport into cells
FUT2	gut/secretor status; influences B12 availability via microbiome dynamics

B) Choline

Gene(s)	Explanation
PEMT / CHDH / BHMT	choline need & use in methylation/membranes

C) Folate

Gene(s)	Explanation
MTHFR / MTHFD1	folate processing and availability

D) Salt sensitivity

Gene(s)	Explanation
ACE / AGT / AGTR1 / REN	RAAS control of fluid and pressure

E) Vitamin D

Gene(s)	Explanation
VDR	receptor response
GC	transport protein
CYP2R1	activation step

F) Caffeine

Gene(s)	Explanation
CYP1A2	primary caffeine metabolism speed
COMT	stimulant sensitivity via catechol handling

G) Fatty acids

Gene(s)	Explanation
FADS1 / FADS2	omega-3/omega-6 conversion efficiency
ELOVL2	long-chain fatty acid elongation
APOA2 / APOA5 / FABP2	dietary fat response/handling

H) Gluten

Gene(s)	Explanation
HLA	celiac-associated immune recognition markers

I) Iron overload

Gene(s)	Explanation
HFE	iron absorption regulation

J) Vitamin C

Gene(s)	Explanation
SLC23A1	vitamin C transport into cells